Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears

Authors

  • Hanife Güler Dönmez Hacettepe University Faculty of Natural & Applied Science Department of Biology, Ankara
  • Şayeste Demirezen Hacettepe University Faculty of Natural & Applied Science Department of Biology, Ankara
  • Sinan Beksaç Hacettepe University, Faculty of Medicine Department of Obstetrics and Gynecology, Ankara

Keywords:

Wnt signaling, Beta-catenin, Cervical smear, Immunocytochemistry

Abstract

OBJECTIVES: The Wnt/β-catenin signaling pathway controls cell fate and homeostasis of embryonic and adult tissues. The aim of the study is to evaluate the activity of Wnt/β-catenin signaling in cervical smears by using immunocytochemistry.
STUDY DESIGN: Cervical smears taken from 200 patients were examined in terms of immunostaining of β-catenin. The membranous staining with moderate (++) or strong (+++) cytoplasmic and/or nuclear staining was scored positive for signaling activity.
RESULTS: Positive Wnt/β-catenin signaling was found in 21 of 200 cases (10.5%). The nucleus was not found to be positive in any of the cells. However, some nuclear membranes were positive. In addition, when signaling was active, the membranous expression of β-catenin was observed to increase by a statistically significant amount epithelial cells (p<0.05).
CONCLUSION: Our data indicates that immunocytochemical techniques can be used to detect the activity of the Wnt/β-catenin signaling pathway in routine cervical smears.

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Published

2013-08-29

How to Cite

1.
Dönmez HG, Demirezen Şayeste, Beksaç S. Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. Gynecol Obstet Reprod Med [Internet]. 2013Aug.29 [cited 2021Apr.15];19(2):91-5. Available from: https://www.gorm.com.tr/index.php/GORM/article/view/193

Issue

Section

Gynecology and Gynecological Oncology

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